FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22 ): 298-300.doi: 10.7506/spkx1002-6300-200922070

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Isolation of Betel Nut Oil for GC-MS Analysis

ZHANG Hai-de,HUANG Yu-lin,HAN Lin   

  1. College of Food Science, Hainan University, Haikou 570228, China
  • Received:2009-06-01 Online:2009-11-15 Published:2010-12-29
  • Contact: ZHANG Hai-de E-mail:zhanghaide@163.com

Abstract:

A method based on silca column chromatograph purification and identification by GC-MS has been developed for the isolation of betel nut oil. The crude extract of betel nut oil dissolved in Petroleum ether was separated by TLC and eluted with cyclohexane-acetone (8:2, V/V) as mobile phase. Results showed that the purified betel nut oil comprised 18 types of fatty acids, among which fourteen acid myristic acid accounted for 26.08%, octadecenoic acid (oleic acid) for 24.20%, eighteen carbon acid (linoleic acid) for 22.70%, hexadecanoic acid (palmitic acid) for 14.09%, and dodecanoic acid (lauric acid) 7.87%. Due to diversity of unsaturated fatty acid, betel nut oil has a great potential to develop for health food with function of regulating blood fat and delaying senility.

Key words: betel nut oil, separating, GC-MS

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